A new study from the University of Vienna, published in the journal iScience, provides the first neural evidence that dogs can distinguish between different human facial expressions, including specific negative emotions. Using fMRI scans, researchers found that happy faces activated the temporal cortex and caudate nucleus — brain regions linked to higher cognitive and reward processing — in eight dogs shown happy versus neutral faces . This suggests happy human faces carry positive emotional significance for dogs . In a second experiment with 12 dogs, machine learning analysis of whole-brain activity revealed distinct patterns that could differentiate fear from anger and sadness, though not anger from sadness . This is the first demonstration that dog brains can distinguish emotions of the same valence . The study's authors caution that the dogs were all pets from loving homes, and street dogs may respond differently . They also note that dogs integrate other cues like body language, voice, and scent when interpreting human emotions . The team plans to investigate how dogs combine these cues and to compare human and canine brain processing of emotional signals .